Isolation, characterization and identification of Actinobacteria of Mangrove ecosystem, Bhitarkanika, Odisha

Abstract

In the present study 15 strains were isolated from the Mangrove ecosystem of Bhitarkanika , Odisha. All the strains have shown white and gray aerial mass colour . Melanoid formation is shown by seven strains and only four strains namely BS, C8, C9 and MB3 shows the distinctive (1) character of reverse side pigments. Spiral spore chain morphology has been shown by 10 strains and only one strain has shown biverticillus spira. Strain BS has shown Rectus form. For the spore surface morphology of three strains SEM analysis is done, among them one strain CS has shown spiny and another two Strain B2 and C11 has shown smooth surface. Assimilation of rhamnose, Inositol and mannitol was better than other carbon sources. Strain B2, C8, C9, MB1, MB2 and MB5 has shown good enzymatic activity. Strain D1 has shown good antimicrobial activity against pathogenic strains of Pseudomonas, E.coli, Klebsiella, Bacillus, and Proteus. Results were interpreted for the identification of strain species from the keys given for 458 species of actinomycetes included in ISP (International Streptomyces Project).