Synthesis and Characterization of ZnO nanoparticles of various sizes and Applications in Biological systems

Abstract

In the present research work, synthesis of zinc nanoparticles and its characterization was
done. In this study, zinc nanoparticles were rapidly synthesized from Zn(NO3)2 solution using soluble starch and formation of nanoparticles observed with in precipitation method.The white synthesized zinc Nanoparticles were characterized by different spectroscopic and analytical techniques such as XRD and SEM. X-ray diraction and SEM analysis studies confirmed the formation of well-dispersed zinc nanoparticles with average particle size to be
in the range of 35 to 41 nm as well as revealed their hexagonal structure. These biologically synthesized nanoparticles were found to be highly toxic against different multi drug resistant human pathogens. Therefore different sized synthesized zinc nanoparticles were used to
study the antimicrobial activity against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus by using well-diffusion and disc diffusion methods and the bacterial growth was monitored by measurement of optical density (OD) of solution
and estimation of colony forming units (CFU) on solid growth medium. The result showed that the antimicrobial activity increased with the decrease of the size of the nanoparticles.