Isolation and Characterization of Peanut (Arachis hypogaea) Lectin

Abstract

Plant lectins which belong to a class of glycoprotein have a wide spectrum of biological significance. In this thesis, the isolation and characterization of the lectins (AHL and PNA) from the peanut (Arachis hypogaea) have been reported. The lactose binding lectins were purified from the peanut seeds by performing affinity chromatography using a lactamyl column. Affinity chromatography products were dialyzed and SDS-PAGE was done to identify the fractions’ molecular weight. Furthermore, Haemagglutination assay was performed for crude,20% cut,60%cut, affinity portion using AB+ve and O +ve blood group.