Das, Chandan Kanta (2012) Isolation and Characterization of Peanut (Arachis hypogaea) Lectin. MSc thesis.
Plant lectins which belong to a class of glycoprotein have a wide spectrum of biological significance. In this thesis, the isolation and characterization of the lectins (AHL and PNA) from the peanut (Arachis hypogaea) have been reported. The lactose binding lectins were purified from the peanut seeds by performing affinity chromatography using a lactamyl column. Affinity chromatography products were dialyzed and SDS-PAGE was done to identify the fractions’ molecular weight. Furthermore, Haemagglutination assay was performed for crude,20% cut,60%cut, affinity portion using AB+ve and O +ve blood group.
|Item Type:||Thesis ( MSc)|
|Uncontrolled Keywords:||peanut lectin, AHL, PNA, Affinity chromatography,SDS-PAGE, Haemagglutination assay|
|Subjects:||Life Science > Biochemistry|
|Divisions:||Sciences > Department of Life Science|
|Deposited By:||Mr Chandan Das|
|Deposited On:||08 May 2012 15:24|
|Last Modified:||08 May 2012 15:24|
|Supervisor(s):||Bhutia, S K|
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