Isolation and Characterization of Peanut (Arachis hypogaea) Lectin

Das, Chandan Kanta (2012) Isolation and Characterization of Peanut (Arachis hypogaea) Lectin. MSc thesis.



Plant lectins which belong to a class of glycoprotein have a wide spectrum of biological significance. In this thesis, the isolation and characterization of the lectins (AHL and PNA) from the peanut (Arachis hypogaea) have been reported. The lactose binding lectins were purified from the peanut seeds by performing affinity chromatography using a lactamyl column. Affinity chromatography products were dialyzed and SDS-PAGE was done to identify the fractions’ molecular weight. Furthermore, Haemagglutination assay was performed for crude,20% cut,60%cut, affinity portion using AB+ve and O +ve blood group.

Item Type:Thesis ( MSc)
Uncontrolled Keywords:peanut lectin, AHL, PNA, Affinity chromatography,SDS-PAGE, Haemagglutination assay
Subjects:Life Science > Biochemistry
Divisions: Sciences > Department of Life Science
ID Code:3074
Deposited By:Mr Chandan Das
Deposited On:08 May 2012 15:24
Last Modified:08 May 2012 15:24
Supervisor(s):Bhutia, S K

Repository Staff Only: item control page