Analysis of dioxygenase gene in marine bacteria involved in polycyclic aromatic hydrocarbon degradation

Mishra, Manisha (2012) Analysis of dioxygenase gene in marine bacteria involved in polycyclic aromatic hydrocarbon degradation. MSc thesis.



The degradation of polycyclic aromatic hydrocarbons (PAHs) by bacteria has been broadly studied. While many pure culture have been isolated and characterize for their ability to grow on PAHs, limited in turn is available on the diversity of microbes involved in PAH degradation in the environment. Pure bacterial cultures were isolated from a highly enriched consortium for biodegradation studies. Bacterial isolates capable of degrading Pyrene and Anthracene were isolated from Paradeep estuary (Odisha) water sample by selective enrichment. Five isolates of pyrene and anthracene degrading pure cultures were obtained, designated as MP-1, MP-4, MP-9, MP-14,MP-18. Isolates were characterized by gram staining, citrate utilization, sugar fermentation, swimming and swarming motility and antibiotic sensitivity test. In 7 days MP-1,MP-4,MP-9,MP-14,MP-18 found to degrade 58.4%, 42.1%, 29.1%, 31.7%, 31.8% at 100 mg/l concentration of Pyrene and 56.3%, 46%, 44.6%, 30.4%, 48.2% at 100mg/l concentration of Anthracene. Polymerase chain reaction (PCR) amplification with PAH-specific primers revealed the presence of a dioxygenase gene in MP-4.The presence of dioxygenase gene may provide opportunities to explore how bacteria develop the abilities to degrade high-molecular-weight polycyclic aromatic hydrocarbons. The ability to profile not only the bacterial community but also the dioxygenases which they encode provides a powerful tool for both assessing bioremediation potential in the environment and for the discovery of novel dioxygenase genes.

Item Type:Thesis ( MSc)
Uncontrolled Keywords:Biodegradation, Pyrene, Anthracene, marine bacteria, dioxygenase gene
Subjects:Life Science > Microbiology
Divisions: Sciences > Department of Life Science
ID Code:3116
Deposited By:mishra manisha
Deposited On:08 May 2012 18:22
Last Modified:02 Sep 2015 19:32
Supervisor(s):Das, Surajit

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