Analysis of catabolic gene in marine bacteria for polycyclic aromatic hydrocarbon degradation

Sahoo, Kirtimayee (2012) Analysis of catabolic gene in marine bacteria for polycyclic aromatic hydrocarbon degradation. MSc thesis.



The aim of the present study was to analyze the biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) compound by the bacterial isolates and localization of the PAH-ring hydroxylating dioxygenase genes. (PAHs) are widely distributed in nature and are known to be toxic and carcinogenic. Some micro-organisms are capable of transforming and degrading PAHs. These potential abilities may be useful in removal of PAHs from the environment. Bacterial isolates capable of degrading Napthalene and Phenanthrene were isolated from Rushikuliya estuary (Odisha) water sample by selective enrichment. The isolates were screened for the ability to degrade Napthalene and Phenanthrene when provided as the sole source of carbon. Five isolates, designated as KR-2, KR-5, KR-7, KR-10 and KR-15 were selected after initial screening. Isolates were characterized by gram staining, citrate utilization, sugar fermentation, swimming and swarmming motility and antibiotic sensitivity test. In 7 days KR-2, KR-5, KR-7, KR-10 and KR-15, found to degrade 50.88%, 50.96%, 50.81%, 50.4% and 50.77% of phenanthrene and 70.01%, 66.01%, 78.18%, 74.1% and 71% of napthelene respectively. Genomic DNA was extracted and ring hydroxylating dioxygenase gene was amplified by primer- Forward primer - (GAG ATG CAT ACC ACG TKG GTT GGA) and Reverse primer - (AGC TGT TGT TCG GGA AGA YWG TGC MGT T). Of the five selected PAH degrading bacteria, KR-2 showed amplification of dioxygenase gene.

Item Type:Thesis ( MSc)
Uncontrolled Keywords:Biodegradation, Phenanthrene, Napthalene, marine bacteria and Dioxygenase gene
Subjects:Life Science > Microbiology
Divisions: Sciences > Department of Life Science
ID Code:3117
Deposited By:sahoo kirtimayee
Deposited On:10 May 2012 17:39
Last Modified:02 Sep 2015 19:36
Supervisor(s):Das, Surajit

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