Development and characterisation of clindamycin hydrochloride loaded PLA/PLGA nanoparticles

Abstract

Clindamycin hydrochloride drug most commonly used as antibiotics in dental and oral infection acts upon various bacterial infections also. In this experiment PLA and PLGA nanoparticle prepared and conjugated with Clindamycin hydrochloride drug. From these two nanoparticles PLGA shows good result. It is found through SEM and Zeta sizer study that these polymers after conjugation with clindamycin hydrochloride gaining mean particular size of 178.6 nm with zeta potential -17.5 mv. Due to very less zeta potential nanoparticles are remain far apart so no clumping found among the drugs. In DSC study it is shown that the Tg (glass transition temperature) of Clindamycin hydrochloride is about 1500C so it take time to disperse inside the body but after conjugation with PLGA its Tg getting reduced to about 480C.Due to this low glass transition temperature it can easily disperse inside the body. After conjugation with Clindamycin hydrochloride there also an investigation done through FTIR studies from which we got that there must be a good conjugation of clindamycin hydrochloride with PLGA nanoparticle. This is because the abundance of OH, C=O, group both are common in PLGA and Clindamycin hydrochloride drug. The stretching band in PLGA-clindamycin hydrochloride is 3644.32 cm-1 refers to the absence of hydrogen bond among the Clindamycin hydrochloride and PLGA which may stands for hydrophobic bond due to much abundance of OH group. At the end it can be tell that after conjugation there is no serious alteration of Clindamycin hydrochloride structure but due to very easily dispersible nature it can shows its bactericidal effect more rapidly in comparison to the conventional medicine which generally takes two to three days.