Cryopreservation of adipose tissue and effect of 17β-estradiol on osteogenesis of omental fat derived stem cells

Abstract

Due to the wide range of applications in reconstructive surgeries and in tissue engineering, the urge to cryopreserve adipose tissue has increased. Cryoprotectants are known to protect the cells from cryoinjury. Investigators across the world are trying to find out ideal cryoprotectants and to develop optimum cryopreservation protocol. However, limited success has obtained yet. This study is a small endeavor to determine the effect various sugars on fat preservation. The cryoprotective action of Mannitol, Sucrose, Trehalose, DMSO, Glycerol, PBS and normal saline have studied. The cryoprotective solutions are prepared in various concentrations and added to fat samples and plunged into liquid nitrogen. The cell viability after post thawing has been evaluated by fat oil ratio and Glycerol 3 phosphate dehydrogenase enzyme activity assay. The results indicated that 5% trehalose, 10% DMSO has shown better cryoprotective action than others. Mannitol has shown the least cryoprotective action in comparison to all the cryoprotectants used in the study. Interestingly, it was found that fat preserved in PBS has good cell viability next to 5%trehalose and 10%DMSO.Omental fat is rich in factors that promote the healing and regeneration. The effect of 17-β-estradiol on the omental adipose derived stem cells during osteogenic differential potential has been studied. The results of the study indicated that 17-β-estradiol enhances the osteogenic differentiation of omental fat derived stem cells.