Development of porcine omentum fat derived extracellular matrix based scaffold and microbeads for soft tissue engineering

Abstract

Trauma, tumor resection, change with age, burns, accidental injury and congenital abnormality causes soft tissue defects or voids within the dermis layer or subcutaneous layer of fat. These defects are due to the loss of fat that is difficult to self-repair. Tissue engineering can be viewed as the best methods for these defects by scaffold, biofilms and microbeads. In this study we prepared scaffold containing porcine adipose tissue derived extracellular matrix by freeze drying and composite microbeads of alginate and extracellular matrix by electrospraying respectively. Adipose tissue was decellularized by physical methods like homogenization, freez e thaw, centrifugation and chemical methods like acetic acid and SDS treatments. Then ECM scaffold was stained with Verhoeff's Van Gieson characterized by SEM, FTIR, DSC, and swelling ratio study. ECM scaffold shown hydrophilic porous structure and having antibacterial activity. The microbeads were characterized by SEM and FTIR. The beads are highly porous containing small microparticles of 7-8 µm.