Unraveling the Signature genes involved in tumors of epithelial and mesenchymal origin

Abstract

Tumors mainly originate from epithelial and mesenchymal cells, majority of which are from epithelial origin. There are certain mechanism like EMT and MET which plays a critical role in malignant tumors or cancer progression. There are various other phenomenon and mechanisms occurring at the molecular and cellular level which are yet to be discovered. Success in unraveling such a mystery can give clue to understand the fatal disease like cancer. Roles of genes involved in various cancers and their expression in different forms of cancer as well as in normal condition can give an insight about cancer. This study could be carried out using microarray analysis. Present work mainly focuses to understand the similarity and dissimilarity between the tumors of epithelial and mesenchymal origin by microarray analysis of differentially expressed genes through Genespring software. Gene ontology analysis was also carried out through Genomatix software and two genes (ABCA8 and SMC4) were selected for experimental validation based upon their regulation and association with cancer. Expressions of these two genes were experimentally validated through qRT-PCR with respect to housekeeping gene beta-actin in the HeLA cell line, a cervical cancer cell line. Studies like this can help us to understand the behavior of different types of cancer at the molecular and cellular level, thereby developing an effective treatment measure for deadly disease like cancer.