Identification of molecular signature in Epithelial tumours

Abstract

Epithelial tumour or carcinoma is the most common cause of death in individual with cancer worldwide. Molecular basis of epithelial tumor is poorly understood. To elucidate the mechanism behind the abstractness of epithelial tumor, “Molecular Signature” is the more accurate and effective than possible standard approach. Microarray data analysis has made it possible to obtain high feature molecular snapshot of genes of an organism at various disease state and experimental conditions. In this study, we discussed the uncovering of molecular signature from epithelial tumor (Brain, Stomach, Cervical cancer) on the basis of relative fold change and potential biomarker ability in cancer. To explore the molecular signature in epithelial tumor, we compared the gene expression profile of brain, stomach, cervical cancer. From microarray analysis we found 201 exclusive set of common genes in epithelial origin tumor and from 201 genes, we are able to identify 10 genes that can be used as molecular signature for all types of cancer which has epithelial origin. Selected two genes (SERPINA3, SH3GL3) were experimentally validated by qRT-PCR in HeLa cell line. qRT-PCR established that these two genes are showing their up regulation with respect to Beta-Actin, which is a housekeeping gene. The identification of molecular signature has promising application for accurate detection, promote early diagnosis and screening of cancer.