Dey, Sandeep (2013) In vitro antitumor potential of soybean lectin, isolated from glycine max. MSc thesis.
Soybean lectin is one of the most important lectins which have anti-cancer activity, primarily confirmed by MTT and trypan blue exclusion assay in various cancer cell lines. The cell viability percentage increases in decreasing dose concentration of the soy lectin. Besides the above it also induces laddering of the DNA in a dose dependent manner. DNA fragmentation was occurred after treating the soya lectin in HeLa cell lines. Isolation of the soya lectin by using Sepharose 4B column is a novel approach. Characterization of this lectin was done by performing Haemagglutination assay and determining molecular weight by both Native and SDS-PAGE which was found to be 120 kD and 30 kD respectively.
|Item Type:||Thesis ( MSc)|
|Uncontrolled Keywords:||Soybean lectin, SBL, Affinity chromatography, SDS-PAGE, haemagglutination assay, MTT Assay, Trypan Blue Exclusion assay|
|Subjects:||Life Science > Cancer Biology|
|Divisions:||Sciences > Department of Life Science|
|Deposited By:||Hemanta Biswal|
|Deposited On:||09 Dec 2013 14:34|
|Last Modified:||09 Dec 2013 14:34|
|Supervisor(s):||Bhutia, S K|
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