production of lignocellulosic ethanol from ipomoea carnea by bacterial cellulase of cow and deer dung

Abstract

Due to rapid growth in population and industrialization, worldwide ethanol demand is increasing continuously lignocellulosic biomass are most abundant and renewable sources of the world they can act as a promising source for bioethanol production. The major objective of the work was to evaluate the effect of acid and steam pretreatment On Ipomoea carnea for improved yield of bioethanol production by using cellulase producing bacteria isolated from Deer and Cow dung and optimization of condition required for maximum activity of cellulase enzyme. Cellulase producing bacteria isolated from Deer and Cow dung screened by congo red test. Cellulase activity was measured by DNS method. From the present study it is concluded that bacteria isolated from deer and cow dung was producing maximum amount of cellulase enzyme after 60 hours (DDB) and after 70 hours (CDB). Cellulase enzyme produced by bacteria isolated from deer and cow dung was found to have pH around 3, temperature 50°C for DDB and 60°C for CDB. Activity of Cellulase enzyme produced by bacteria isolated from deer and cow dung was found to be increased by the addition of 5 mM MnSO4 ( both DDB and CDB). It Possible to produce lignocellulosic bioethanol (5.52%) from Ipomoea carnea after steam and acid pretreatment by using Cellulase for Saccharification (72 hours) and Saccharomyces cerevisiae for fermentation (72 hours). Bioethanol from lignocellulosic biomass is a globally accepted alternative fuel. The production of ethanol from Ipomoea carnea would have the dual advantage of producing energy and serving as an effective method of weed management