Potential Applications of Biosurfactant from Marine Bacteria in Bioremediation

Mohanty , Manorama (2013) Potential Applications of Biosurfactant from Marine Bacteria in Bioremediation. MSc thesis.



Marine bacteria were screened for their ability to synthesize biosurfactants which can effectively degrade polycyclic aromatic hydrocarbons (PAHs) as the sole carbon and energy source. PAHs are very harmful to flora and fauna having many toxic effects and also affecting mankind adversely. The main objective of this work was to study the potential applications of biosurfactant in aerobic degradation of PAHs in stress conditions. The antimicrobial and anti-adhesive studies of the biosurfactant were also tested against different pathogenic strains. Marine bacteria were isolated from sediment samples of Paradip Port, Visakhapatnam Port, Rishikulya, Bhitarakanika and screened for their biosurfactant production and also growth was optimised in carbon and nitrogen sources for the best biosurfactant production. Naphthalene and Phenanthrene (PAHs) degrading isolates were evaluated for their biodegradative potential under laboratory scale through UV-Vis spectroscopy, phenotypical characterization by SEM studies. Five potent isolates JV201, JV501, JV502, JP011, and JP022 were identified to be Ochrobactrum, Streptococcus, Pseudomonas sp., Pseudomonas aeruginosa and Achromobacter xylosoxidans having 99.9%, 99.6%, 99%, 99.3%, 98.6% of degradation of Phenanthrene (100mg/l) and 99%, 99.1%, 89.75%, 94.01%, 97.02% of degradation of Naphthalene (100 mg/l) respectively having good antimicrobial and anti-adhesive properties.

Item Type:Thesis ( MSc)
Uncontrolled Keywords:Biosurfactants; PAHs (polycyclic aromatic hydrocarbons); Bioremediation
Subjects:Life Science > Microbiology
Life Science > Environmental Science
Divisions: Sciences > Department of Life Science
ID Code:5397
Deposited By:Hemanta Biswal
Deposited On:19 Dec 2013 10:52
Last Modified:02 Sep 2015 19:32
Supervisor(s):Das, Surajit

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