Biofabrication and extraction of nanoparticles from seeds of Syzygium cumini

Abstract

The novel strategies are applied for synthesis of silver nanoparticles (AgNP) using biological methods, since AgNP has various applications including high anti-microbial activity. The biological method using plant extracts is relatively unexplored. Nanoparticles formed from plants are more stable and bio-compatible. For the project, Syzygium cumini seeds extract is used to fabricate extracellular AgNP. The ionic form of silver is reduced to its elemental form by the bioreduction reaction of silver nitrate by components present in seed extract like flavonoids, phytochemicals and alkaloids. The reduced elemental silver was further capped by the moieties present in extract into nanoparticle sizes and shapes. The sizes were further characterized for chemical, physical characteristics AgNP, using UV-Vis Spectroscope, Dynamic Light Scattering (DLS), Zeta potential, X-ray diffraction (XRD), and Scanning Electron Microscope (SEM). The morphological properties of the nanoparticles formed were spherical in shape, polydisperse, and negatively charged. Size-selective purification of the nanoparticles was done by centrifugation, filtration and chromatographic methods. In chromatographic method, elution’s were collected and further characterized using UV-Vis spectroscope and Attenuated Total Reflection-Fourier Transform infrared spectroscope (ATR-FTIR).