Study of expression of p15PAF and interaction with miR-429 in fibrosarcoma cell line HT-1080

Abstract

MicroRNAs are a family of small, non-coding RNAs which regulate gene expression at post-transcriptional level. These ncRNAs influence cellular physiology by directly interacting with target gene transcripts. miRNA expression pattern can be correlated with cancer types, stages and other clinical variables. Therefore miRNA profiling can be used as a tool for cancer diagnosis and prognosis in almost all aspects of cancer biology such as proliferation, apoptosis, invasion and angiogenesis. Our study aims at identification of novel miRNA-mRNA target pairs that are hypothesized to play role in fibrosarcoma through an interaction map analysis from microarray data followed by experimental validation of selected pair of mRNA and miRNA by qRT-PCR. The target interaction map analysis revealed one novel target pair i.e. hsa-miR-429- p15PAF which can be potential therapeutic target in fibrosarcoma.