In silico genome analysis and finding a target protein for mycobacterium tuberculosis (H37Rv)

Abstract

Mycobacterium tuberculosis (Mtb) is the bacteria for causing TB in human. H37Rv strain is responsible for this disease. Among the bacterial infectious diseases it is the leading cause of deaths in the world. According to statics it kills 30000 people per year in India. Multi-drug-resistant tuberculosis (MDR TB) shows resistance against these effective drugs- isoniazid and rifampicin. In this approach we have found certain target protein which can give rise to novel drug for this disease. All the metabolic pathways which are not present in the human but present in pathogen are taken as unique pathways. Here there are five such pathways which unique and are present only in bacteria. The genes responsible for these unique pathways were listed and were analyzed for their essentiality for the survival of the pathogen from the DEG (Database of Essential Genes). The essential genes were then blasted against the human genome through BLASTP. The structures and functions of non-homologous proteins were then analyzed.