Fabrication and extraction of silver nanoparticle using Acorus calamus

Abstract

Nanotechnology is a rising field of science and technology that deals with the particles having a minimum of one dimension in 1 to 100 nm. Nanotechnology gives rise to nanomaterials as its product. Nanomateials can be nanoparticles, nanorods, nanospheres ,nanocylinders,etc. Ag nanoparticles has many properties like antifungal activity, antibacterial activity, optical properties, conductive properties,etc. Due to its demand of diversified use, we have fabricated Ag nanoparticle using rhizome extract from Acorus calamus. The fabrication is followed by characterisation of Ag nanoparticles using UV-vis spectroscope, FTIR, DLS, ZETA and SEM. The characterisation results in spherical shaped Ag nanoparticle approximately equal to the nano range. After fabrication the reduced Ag nanoparticle is extracted from the colloidal solution using Size exclusion chromatography. After extraction again characterisation is done,which shows that the size reduces after extraction and is more stable with a high zeta potential. Acorus calamus is such a biological enhancer that is capable of producing quality and effective Ag nanoparticles that can serve the mankind in various way and as well as give us a safer and healthier environment.