fabrication of electrospun dicalcium phosphate anhydrous and chitosan based scaffolds

Abstract

Dicalcium phosphate anhydrous (DCPA) powder was synthesized in the size range between 200- 750 nm using calcium carbonate and phosphoric acid in ethanol solution. The X-ray diffraction pattern of the synthesized powder showed monetite as the major phase with some unreacted calcium carbonate as secondary phase. After 2hrs of stirring on reaction mixture in ethanol 3Wt% of each chitosan, poly ethylene oxide, dicalcium phosphate anhydrous was used for fabricating electrospun fibrous scaffold for bone tissue engineering. Glacial acetic acidcounts to be a suitable solvent for electrospining the suspension. The fabricated electrospun scaffold was characterized using X-ray diffraction and Fourier Transform infrared spectroscopy. Both X-ray diffraction and Fourier Transform infrared spectroscopy confirm the presence of chitosan, dicalcium phosphate and hydroxyapatite as major phases in the scaffold.Nanofibre morphology of the scaffold with average fiber diameter varying between 61 nm to 703 nm was observed from scanning electron micrograph of the electrospun scaffold.