Study of Microrna-Mediated Regulation of Metastasis in Fibrosarcoma

Abstract

microRNAs (miRNAs) are the small non-protein-coding RNA,Regulate target gene expression via transcriptional degradation or translational repression.In this study our aim was to investigate the role of miR-429 in metastasis process in fibrosarcoma and its possible mode of regulation. Microarray data analysis for fibrosarcoma, is trying to screen out the probable miRNAs and genes responsible for metastasis. After gating a novel pair of gene and miRNA, Experimental validation of selected gene and miRNA expression was carried out through qRT-PCR in fibrosarcoma cell line HT1080, as compare to normal fibroblast cell line WI-38.The role of miR-429 in metastasis was further accessed via over-expression of the miRNA in HT1080 and carrying out during in-vitro metastasis detection assay like cell migration, cell proliferation assays, By using different assay we found that miR-429 expression is decreased in Fibrosarcoma where it might be acting as tumor suppressor in cellular model, Where it prevents tumor development by negatively inhibiting oncogene KIAA0101 that induce cell proliferation, cell migration. But Over expression of miR-429 inhibits the cell proliferation and cell migration which might be helping in metastasis and ultimately contributing to lethality of Fibrosarcoma.