Apoptosis and Autophagy Induction in T98G Cells (Glioblastoma) with Concanavalin A

Abstract

Concanavalin A (Con A) is a lectin with mannose/glucose binding specificity depicts variety of biological science functions such as, study of hormones/receptor, mitogenic T-cell activation, characterizing normal and malignant cells as well as positive or negative staining bacteria, as lectin affinity chromatography, as an immobilization matrix, etc. The present study evaluated the potential role of Concanavalin A, anti proliferative effect in glioblastoma cell line i.e. T98G cells. The lectin Con A was isolated by salting-in and salting-out procedure and purified by affinity chromatography using sepharose 4B column. The protein was estimated and lyophilized for the in-vitro study of Con A. Initially, the cell viability assay (MTT assay) was done in a normal and the cancer cell for in-vitro study, IC-50 value was determined. Based on this, apoptosis and autophagy were studied using DAPI staining, Annexin V FITC staining, western blotting, Caspase 3/7 Glo assay, Acridine orange staining and GFP-LC3 transfection. In conclusion, we demonstrated that Con A induces both apoptosis and autophagy in a dose dependent manner in glioblastoma. Review of literature shows the molecular mechanism in hepatoma and Hela cells, but it is unexplored in T98G cell. Hence as the novelty of work we tried to explore the anti-proliferative activity of Con A in glioblastoma cell. Its molecular mechanism can be further studied for clinical treatment of cancer.