Characterization of Biofilm Forming Acidotolerant Lysinibacillus sp. RTA 01 and its Utilization in Biosynthesis of Chromium Oxide Nanoparticles

Abstract

In this rapidly developing world with the increase in development there is increase in the toxic waste also. The problem has become so alarming that the toxic waste management requires an urgent attention. Although many conventional techniques has been utilized for waste remediation but recently developed nanotechnology based remediation process has strengthen the remediation process. Here we report the ability of Lysinibacillus sp. RTA 01 isolated from contaminated sites for the biological synthesis of chromium oxide (Cr2O3) nanoparticles (NPs) which provide us for the development of clean, non-toxic and environment friendly procedure for NPs synthesis. The synthesized NPs from different parts of isolate like cell pellet, supernatant and EPS were characterized by UV Visible spectrophotometer, XRD (X-Ray Diffraction), FTIR (Fourier Transform Infra-red Spectroscopy), FESEM (Field Emission Scanning Electron Microscope), for confirming the formation and size of NPs. Zeta analysis was also done for determining the stability of the synthesized NPs. The bacterial isolate showed more than 50% reduction of hexavalent chromium (Cr6+) to trivalent chromium (Cr3+) when incubated with media supplemented with K2Cr2O7 solution. The reduction of toxic Cr6+ into stabilized Cr3+ NPs by acidotolerant Lysinibacillus sp. RTA 01 showed the large scale industrial application in successful detoxification of contaminated sites in an environmentally sustainable way.