Nano-Hydroxyapatite and Bone Derived Extracellular Matrix Biomimetic Composite Coating On 316L Stainless Steel

Abstract

In this project 316L stainless steel samples were polished, chemical treated and were coated with synthesized nano hydroxyapatite by modified electrophoretic deposition method consisting of coating the same sample multiple times to reduce cracks. Extracellular matrix from bovine bone was extracted and was used to synthesize biomimetic nano-hydroxyapatite and bone derive extracellular matrix composites. These were then used to coat the steel samples on top of the base nano hydroxyapatite coating. The base coat, the bone derived extracellular matrix and the nano-hydroxyapatite and bone derive extracellular matrix composites were characterized. The synthesized nano-hydroxyapatite were found to be nano rod in shape. The extracellular matrix had an 82% collagen content as well as other essential non-collagen proteins. The composites showed a significant higher cell proliferation than the hydroxyapatite.