Bagh, Sangram Keshari (2015) Impact of Ammonium Based Ionic Liquids on the Conformation
of Bovine serum albumin (BSA). MSc thesis.
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Abstract
In this study, impact of different ammonium based ionic liquids on the structure of Bovine serum albumin (BSA) was investigated by different spectroscopic methods like, UV-vis, fluorescence and CD spectroscopy. Fluorescence results reveal that the ILs has no significant impact on the environment of tryptophan residues. From the Far-UV CD results, it was observed that these ILs increase the α-helicity of the protein, BSA. Near-UV CD study confirms the fluorescence results whereas it shows the alteration of tertiary structure of BSA especially around disulphide bonds.
| Item Type: | Thesis ( MSc) |
|---|---|
| Uncontrolled Keywords: | BSA, Conformational Change, Ionic liquids, Fluorescence, Circular Dichroism, UV-Vis Spectroscopic Studies |
| Subjects: | Chemistry > Physical Chemistry |
| Divisions: | Sciences > Department of Chemistry |
| ID Code: | 7929 |
| Deposited By: | Mr. Sanat Kumar Behera |
| Deposited On: | 06 Jul 2016 12:16 |
| Last Modified: | 06 Jul 2016 12:16 |
| Supervisor(s): | Sahoo, H |
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