Isolation of Bacteria from Coal Mine Dust with Metal Nanoparticle Fabrication Ability

Abstract

Nanoparticles being the smallest unit of nanotechnology are playing important roles in various fields. There are different kinds of nanoparticles ranging from metals to non-metals, and also from carbon sources. Though various methods have been optimized for synthesis of nanoparticles, still biological methods for synthesis of metal and metal oxide nanoparticles have drawn attention of different research groups due to their advantageous properties over physical and chemical methods. The objective of this study is to synthesize silver nanoparticles using bacteria isolated from coal mine. These bacteria are chosen assuming that they have beter quality for metal tolerance than normal bacteria. The silver nanoparticles are synthesized at the MIC value of AgNO3 against the isolated bacteria. The characterization of nanoparticles samples were done using different techniques like UV-Vis spectroscopy, DLS, FE-SEM, and ATR-FTIR proved that they are silver nanoparticles with coating of different bacterial moieties like proteins, carbohydrates, etc.