Investigation of Antimicrobial Potential of Nisin Against Bacillus thuringiensis

Abstract

Antibiotics have major roles in improvement of human and animal health against bacterial diseases. But now a days many bacterial pathogens show resistance to antibiotics due to high amount of use of these antibiotics. It is necessary to overcome this problem by think about new strategies to kill or inhibit the bacteria. Here we introduced nisin an antimicrobial peptide 34 amino acid sized produced by lactococcus lactis which is highly active against Gram positive bacteria. Discoveries of Lipid II which is a target for nisin and in particular, the important role played by the pyrophosphate group and brought nisin to the forward in the battle with resistant human infections as a model case for the production of new antibiotics. Recent study on Bacillus thuringiensis concluded that high amount of Bt toxin is toxic to human as well as animal.Here we evaluated the effect of antimicrobial activity ofNisin on Bacillus thuringiensisby determining the MIC (Minimum inhibitory concentration), Zeta potential (electrokinetic potential), electron microscopy and bacterial cell viability by Baclightassay in the presence and absence of Ampicillin. We observed that with the increasing concentration of nisin prolonged lag phase and in combination with ampicillin skip the lag phase of Bacillus thuringiensis. The electron micrograph structure showed clumping and blebs of bacteria with increasing concentration of nisin.