Estimation of Kinetic Parameters for Production of PHB

Abstract

High use of plastics lead to environmental pollution as this undergoes a very slow process of degradation. To eradicate this problem there is a demand of bioplastics. Polyhydroxyalkanoates (PHAs) are bioplastics with most common form as Polyhydroxybutyrates. In this study, Bacillus subtilis is used to produce PHB using LB broth media. Glucose is used as carbon source and Ammonium sulphate is used as nitrogen source. The cells were cultured in optimal conditions with 37°C temperature, 200rpm in shaking flask incubator. With the estimation of PHB, the biomass formed and glucose utilized were also analyzed. With the references of the plots drawn with the data obtained, different kinetic parameters like μ, Ks, Ki, K1, K2 were determined.