Production of Bacoside A- A Potent Herbal Drug for Alzheimer in Callus Cultures of Bacopa Monnieri(L.) Pennell

Abstract

Alzheimer’s disease (AD) is a chronic and progressive neurodegenerative disease .An important approach for treating AD is the inhibition of acetylcholine esterase (AChE) and Monoacylglyceride Lipase (MAGL). Many of the medicinal plants have suggested for treatment of Alzheimer. But only few plants only proved for their Anti-Alzheimeric potential. One such herb is Bacopa monnieri. . Bacosides are most active constituents of Bacopa monnieri. Bacoside A , mostly reported herbal drug- a mixture of four triglycosidic saponins, namely bacoside A3, bacopaside II, bacopasaponin C and the jujubogenin isomer. Its inhibiting potential were analysed by molecular docking. It is having very low binding energy of -20.1 kcal/mol and -12.7 kcal/mol on the targets AChE and MAGL respectively. The druglikeness and toxicity were predicted with ADMET predictor tools namely molinspiration and protox respectively. Bacoside comes under class IV toxicity. This project related to the production of Bacoside A in Callus Cultures of Bacopa monnieri and its enhancements through Elicitation. Elicitor may be defined as a substance which, when introduced in small concentrations to a living cell system, initiates or improves the biosynthesis of specific compounds. Callus of Bacopa monnieri was efficiently induced by 0.5mg/l of NAA. supplement of Auxins IAA was not much efficient in callus induction. Cytokinin BAP was used to inhibit the root formation. The developed fried callus was used for suspension culture under optimised parameters. The suspension cultures were treated under the influence of the Elicitors. HPLC was used for estimation of Bacoside A.