Development of nanoparticle-polymer based composite films
and its effect on bacterial biofilm formation

Abstract

Antimicrobial surfaces and materials are being emerging to control infectious microorganisms and are important in our day to day life and effectually protect the public health from pollutants including organic, inorganic and biological substances in wide range forms. Developing hybrid nanocomposites coatings that exhibit multiple approach of preventing biofilm formation provides a new generation material to combat against sessile bacterial community. In this study, we fabricated a hybrid nanoparticles (silver nanoparticles-AgNPs, Graphene oxide-GO) and polymer base (chitosan-Ch) which shows both the antibacterial and anti-adhesion properties to prevent and kill the bacteria. The fabrication and characterization of the various composite films GO-Ch, GO-AgNPs, Ch-AgNPs and GO-Ch-AgNPs were analyzed by UV–visible spectroscopy, Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM) and Fourier transform Infrared spectroscopy (FTIR). The antibiofilm activity is evaluated against two model bacteria relative to composite. The silver impregnated nanocomposites exhibit higher antibacterial activity by capture killing process and GO-Ch film exhibit anti biofilm activity by anti-adhesion activity. The results obtained in this research provided a potential next generation composite coating against prevention of biofilms.