Isolation and characterization of marine bacteria for value-added products

Gupta, Kriti (2018) Isolation and characterization of marine bacteria for value-added products. MTech thesis.

[img]PDF (Restricted upto 31/03/2021)
Restricted to Repository staff only

1675Kb

Abstract

Isolation and characterization of marine bacteria for value-added products Marine micro-organisms are attaining great significance in the field of biotechnology. The present study focuses on isolation of protease producing and ethanol-producing bacteria from marine samples. Total four protease producing strains (Dpro-1,Dpro-2, Dpro-3, and Dpro-4)and ten ethanol-tolerant strains were isolated. Out of the four protease producing strains,Dpro-1 gave the best proteolytic activity of 0.117U/ml and was identified as Bacillus subtilis.Upon optimization, the protease activity increased approximately 100%. For the tenethanol-tolerant strains, different domestic wastes (paper, kitchen, garden, and fruit pulp)were used as the substrate. The bacterial strain E4 in the kitchen waste and the fruit pulp waste provided the best outcome for ethanol production via colorimetric assay. The ethanol estimation via HPLC showed a small peak.Hence,it was resolved that the selected isolates are ethanol producing,but for better analysis,confirmation through gas chromatography is needed.

Item Type:Thesis (MTech)
Uncontrolled Keywords:Marine bacteria; Protease; Optimization; Ethanol; Domestic wastes
Subjects:Engineering and Technology > Biomedical Engineering
Divisions: Engineering and Technology > Department of Biotechnology and Medical Engineering
ID Code:9482
Deposited By:IR Staff BPCL
Deposited On:28 Mar 2019 12:04
Last Modified:28 Mar 2019 12:06
Supervisor(s):Sarkar, Angana

Repository Staff Only: item control page